Among microbial expression hosts, Bacillus has carved out a unique niche in biomanufacturing. Unlike Gram-negative bacteria such as E. coli, Bacillus subtilis and related species are naturally endotoxin-free, simplifying downstream purification and expanding their appeal for therapeutic proteins, enzymes, and next-generation biologics. For a Contract Development and Manufacturing Organization (CDMO), mastery of Bacillus systems requires balancing its advantages—secretion capability, GRAS status, industrial […]