High-density bioprocessing from 50 mL shake-flask to 40,000 L cGMP suites.
Quick Facts
- Pilot Scale: 50 L → 2,000 L stainless-steel GMP bioreactors
- Commercial Scale: 4 × 7,500 L up to 8 × 37,500 L
- Modes: Batch, fed-batch, and continuous—configurable per project
- Proof-of-Concept: Rapid screening in small‐scale bioreactors
- Optimization: Media and process tuning in 5 L → 2,000 L systems
Integrated Workflow
- Strain On-Boarding
CRISPR-edited E. coli, Pichia, or GRAS fungi enter a 1 L bench reactor; baseline CPP/CQA map delivered in 48 h. - Media & Feed Strategy Design
DoE screens carbon/nitrogen ratios, trace metals, and pH windows; ML regression models predict optimal feed curves. - Scale-Up Train
1 L → 10 L → 200 L → 2,000 L → 20,000 L, each step matched for k<sub>La</sub> and shear to preserve cell physiology. - Real-Time Control
Inline sensors stream to a Part 11 historian; PID algorithms adjust glucose, methanol, or glycerol feeds every 30 s. - Harvest & Hold
Rapid cooling (to 5–15 °C) plus pH shift suppresses proteases; clarified broth handed off downstream in < 30 min.
Why Aquaterra?
- Density without Drift – Consistently achieve OD₆₀₀ > 100 or DCW > 100 g/L while maintaining target titers.
- Sensor-Driven Efficiency – Soft-sensor feedback minimizes over-feeding and cuts raw-material costs.
- Compliance Built-In – Every fermentor skid is FDA & EMA validated—no retroactive paperwork.
- Speed to Data – Bench-to-200 L reproducibility proven in < 1 month, giving sponsors earlier “go/no-go” clarity.
Next Step
Need microbial runs that scale cleanly and pass every audit?
Email info@aquaterrabiotech.com to book a detailed process walkthrough or reserve fermentor slots this quarter.