From first construct to GMP-ready master cell bank—fast, traceable, and built to scale.
Quick Facts
- CHO-K1, CHO-ZN, HEK293, Pichia—four plug-and-play platforms
- 96 → 1 536 clones screened per campaign (AI-ranked hit list)
- ≤ 10 days from transfection to copy-number-verified pools
- > 95 % genetic stability across 60 doublings
- Up to 10 g L-¹ titer in 14-day fed-batch feasibility runs
Integrated Workflow
- Vector & Construct Design
Codon-optimized gene, synthetic introns, and secretion tags finalized and QC-sequenced in < 48 h. - Genome Insertion / Transfection
CRISPR/Cas9 knock-ins or high-efficiency electroporation; single-use closed systems cut contamination risk to near zero. - Pool Generation & High-Throughput Screening
AI-guided imaging + FACS sort top-2 % producers; Octet titer assay confirms hits the same day. - Clonal Isolation & Mini-Bioreactor Ranking
24-deep ambr® 15 and 250 mL DASbox runs map CPP/CQA space and predict full-scale kinetics. - Stability & Productivity Verification
60-generation passaging, Southern blot / ddPCR copy-number checks, and glycan profiling lock candidate. - Master & Working Cell Banks
GMP fills in ISO 5 isolators; full sterility, mycoplasma, and adventitious-virus panels included.
Why Aquaterra?
- Days, Not Months – Lab-to-verified pools in ≤ 10 days; clone finalization typically ≤ 8 weeks.
- AI-Ranked Picks – Machine-vision scoring cuts false positives by 70 %, focusing resources on true high producers.
- Regulatory-Ready – Every banking run generates a CTD-aligned dossier—no backfill paperwork.
- Built for Scale – Mini-bioreactor data feeds digital twins that mirror shear and k<sub>La</sub> at 2 000 L and beyond.
Next Step
Need a cell line that scales cleanly and wins first-pass regulatory review?
Email info@aquaterrabiotech.com to arrange a project scoping call or reserve engineering slots this quarter.